Record of an alleged Solitary Eagle in Oaxaca is a Great Black Hawk

In a recent article in this journal (García-Grajales et al., 2018), an adult Great Black Hawk (Buteogallus urubitinga) was mistakenly identified as an adult of the Solitary Eagle (Buteogallus solitarius). The Solitary Eagle differs from the Greater Black Eagle in having the longest and widest wings, and the shortest tail. These characteristics give it a more triangular shape in flight. These differences are easy to see in Figures 1–3. The same authors also cite a case of the nesting of the Solitary Eagle in Mexico (Smith, 1982). However, after reviewing photos of the young, we consider that this record is probably the Common Black Hawk (B. anthracinus). The illustrations of the Solitary Eagle in field guides of Mexico and Central America (Howell & Webb, 1995; Van Perlo, 2006) demonstrate greater similarity with the Great Black Hawk, contributing to the frequent confusion of the two species if used without consulting the text. The new features published here and in Clark et al. (2006) and Clark and Schmitt (2017) should help with correct identification of Buteogallus species in the future.En un artículo reciente en esta revista (García-Grajales et al., 2018), un adulto del Aguililla Negra Mayor (Buteogallus urubitinga) fue identificado erróneamente como un adulto del Águila Solitaria (Buteogallus solitarius). El Águila Solitaria difiere del Aguililla Negra Mayor en tener las alas más largas y anchas, y la cola más corta. Estas características le confieren una forma más triangular en el vuelo. Estas diferencias son fáciles de apreciar en la Figuras 1–3. Los mismos autores también citan un caso del anidamiento del Águila Solitaria en México (Smith, 1982). Sin embargo, después de revisar fotos de las crías, consideramos que este registro probablemente se trata del Aguililla Negra Menor (B. anthracinus). Las ilustraciones del Águila Solitaria en guías de campo de México y Centroamérica (Howell & Webb, 1995; Van Perlo, 2006) demuestran mayor similitud con el Aguililla Negra Mayor, contribuyendo a la confusión frecuente de las dos especies si se utilizan sin consultar el texto. Las nuevas características publicadas aquí y en Clark et al. (2006) y Clark y Schmitt (2017) deben ayudar a su correcta identificación en el futuro

Quantification of Hair Cortisol Concentration in Common Marmosets (\u3cem\u3eCallithrix jacchus\u3c/em\u3e) and Tufted Capuchins (\u3cem\u3eCebus apella\u3c/em\u3e)

Quantifying cortisol concentration in hair is a non-invasive biomarker of long-term hypothalamic-pituitary-adrenal (HPA) activation, and thus can provide important information on laboratory animal health. Marmosets (Callithrix jacchus) and capuchins (Cebus apella) are New World primates increasingly used in biomedical and neuroscience research, yet published hair cortisol concentrations for these species are limited. Review of the existing published hair cortisol values from marmosets reveals highly discrepant values and the use of variable techniques for hair collection, processing, and cortisol extraction. In this investigation we utilized a well-established, standardized protocol to extract and quantify cortisol from marmoset (n = 12) and capuchin (n = 4) hair. Shaved hair samples were collected from the upper thigh during scheduled exams and analyzed via methanol extraction and enzyme immunoassay. In marmosets, hair cortisol concentration ranged from 2710 – 6267 pg/mg and averaged 4070 ± 304 pg/mg. In capuchins, hair cortisol concentration ranged from 621 – 2089 pg/mg and averaged 1092 ± 338 pg/mg. Hair cortisol concentration was significantly different between marmosets and capuchins, with marmosets having higher concentrations than capuchins. The incorporation of hair cortisol analysis into research protocols provides a non-invasive measure of HPA axis activity over time, which offers insight into animal health. Utilization of standard protocols across laboratories is essential to obtaining valid measurements and allowing for valuable future cross-species comparisons

OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation

We describe the ability of an engineered glycosyltransferase (OleD Loki) to catalyze the N‐glycosylation of tertiary‐amine‐containing drugs and trichostatin hydroxamate glycosyl ester formation. As such, this study highlights the first bacterial model catalyst for tertiary‐amine N‐glycosylation and further expands the substrate scope and synthetic potential of engineered OleDs. In addition, this work could open the door to the discovery of similar capabilities among other permissive bacterial glycosyltransferases

Swimming Against the Flow: Environmental DNA Can Detect Bull Sharks (\u3ci\u3eCarcharhinus leucas\u3c/i\u3e) Across a Dynamic Deltaic Interface

© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd Human activities in coastal areas are accelerating ecosystem changes at an unprecedented pace, resulting in habitat loss, hydrological modifications, and predatory species declines. Understanding how these changes potentially cascade across marine and freshwater ecosystems requires knowing how mobile euryhaline species link these seemingly disparate systems. As upper trophic level predators, bull sharks (Carcharhinus leucas) play a crucial role in marine and freshwater ecosystem health. Telemetry studies in Mobile Bay, Alabama, suggest that bull sharks extensively use the northern portions of the bay, an estuarine–freshwater interface known as the Mobile-Tensaw Delta. To assess whether bull sharks use freshwater habitats in this region, environmental DNA surveys were conducted during the dry summer and wet winter seasons in 2018. In each season, 5 × 1 L water samples were collected at each of 21 sites: five sites in Mobile Bay, six sites in the Mobile-Tensaw Delta, and ten sites throughout the Mobile-Tombigbee and Tensaw-Alabama Rivers. Water samples were vacuum-filtered, DNA extractions were performed on the particulate, and DNA extracts were analyzed with Droplet Digital™ Polymerase Chain Reaction using species-specific primers and an internal probe to amplify a 237-base pair fragment of the mitochondrial NADH dehydrogenase subunit 2 gene in bull sharks. One water sample collected during the summer in the Alabama River met the criteria for a positive detection, thereby confirming the presence of bull shark DNA. While preliminary, this finding suggests that bull sharks use less-urbanized, riverine habitats up to 120 km upriver during Alabama\u27s dry summer season

A single center phase II study of ixazomib in patients with relapsed or refractory cutaneous or peripheral T‐cell lymphomas

The transcription factor GATA‐3, highly expressed in many cutaneous T‐cell lymphoma (CTCL) and peripheral T‐cell lymphomas (PTCL), confers resistance to chemotherapy in a cell‐autonomous manner. As GATA‐3 is transcriptionally regulated by NF‐κB, we sought to determine the extent to which proteasomal inhibition impairs NF‐κB activation and GATA‐3 expression and cell viability in malignant T cells. Proteasome inhibition, NF‐κB activity, GATA‐3 expression, and cell viability were examined in patient‐derived cell lines and primary T‐cell lymphoma specimens ex vivo treated with the oral proteasome inhibitor ixazomib. Significant reductions in cell viability, NF‐κB activation, and GATA‐3 expression were observed preclinically in ixazomib‐treated cells. Therefore, an investigator‐initiated, single‐center, phase II study with this agent in patients with relapsed/refractory CTCL/PTCL was conducted. Concordant with our preclinical observations, a significant reduction in NF‐κB activation and GATA‐3 expression was observed in an exceptional responder following one month of treatment with ixazomib. While ixazomib had limited activity in this small and heterogeneous cohort of patients, inhibition of the NF‐κB/GATA‐3 axis in a single exceptional responder suggests that ixazomib may have utility in appropriately selected patients or in combination with other agents.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/139920/1/ajh24895.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/139920/2/ajh24895_am.pd

Entropic Tension in Crowded Membranes

Unlike their model membrane counterparts, biological membranes are richly decorated with a heterogeneous assembly of membrane proteins. These proteins are so tightly packed that their excluded area interactions can alter the free energy landscape controlling the conformational transitions suffered by such proteins. For membrane channels, this effect can alter the critical membrane tension at which they undergo a transition from a closed to an open state, and therefore influence protein function \emph{in vivo}. Despite their obvious importance, crowding phenomena in membranes are much less well studied than in the cytoplasm. Using statistical mechanics results for hard disk liquids, we show that crowding induces an entropic tension in the membrane, which influences transitions that alter the projected area and circumference of a membrane protein. As a specific case study in this effect, we consider the impact of crowding on the gating properties of bacterial mechanosensitive membrane channels, which are thought to confer osmoprotection when these cells are subjected to osmotic shock. We find that crowding can alter the gating energies by more than $2\;k_BT$ in physiological conditions, a substantial fraction of the total gating energies in some cases. Given the ubiquity of membrane crowding, the nonspecific nature of excluded volume interactions, and the fact that the function of many membrane proteins involve significant conformational changes, this specific case study highlights a general aspect in the function of membrane proteins.Comment: 20 pages (inclduing supporting information), 4 figures, to appear in PLoS Comp. Bio

Low-Temperature Specific Heat of an Extreme-Type-II Superconductor at High Magnetic Fields

We present a detailed study of the quasiparticle contribution to the low-temperature specific heat of an extreme type-II superconductor at high magnetic fields. Within a T-matrix approximation for the self-energies in the mixed state of a homogeneous superconductor, the electronic specific heat is a linear function of temperature with a linear-$T$ coefficient $\gamma_s(H)$ being a nonlinear function of magnetic field $H$. In the range of magnetic fields H\agt (0.15-0.2)H_{c2} where our theory is applicable, the calculated $\gamma_s(H)$ closely resembles the experimental data for the borocarbide superconductor YNi$_2$B$_2$C.Comment: 7 pages, 2 figures, to appear in Physical Review

Extensive HST Ultraviolet Spectra and Multi-wavelength Observations of SN 2014J in M82 Indicate Reddening and Circumstellar Scattering by Typical Dust

SN 2014J in M82 is the closest detected Type Ia supernova (SN Ia) in at least 28 years and perhaps in 410 years. Despite its small distance of 3.3 Mpc, SN 2014J is surprisingly faint, peaking at V = 10.6 mag, and assuming a typical SN Ia luminosity, we infer an observed visual extinction of A_V = 2.0 +/- 0.1 mag. But this picture, with R_V = 1.6 +/- 0.2, is too simple to account for all observations. We combine 10 epochs (spanning a month) of HST/STIS ultraviolet through near-infrared spectroscopy with HST/WFC3, KAIT, and FanCam photometry from the optical to the infrared and 9 epochs of high-resolution TRES spectroscopy to investigate the sources of extinction and reddening for SN 2014J. We argue that the wide range of observed properties for SN 2014J is caused by a combination of dust reddening, likely originating in the interstellar medium of M82, and scattering off circumstellar material. For this model, roughly half of the extinction is caused by reddening from typical dust (E(B-V ) = 0.45 mag and R_V = 2.6) and roughly half by scattering off LMC-like dust in the circumstellar environment of SN 2014J.Comment: 17 pages (excluding references and tables), 15 figures, accepted to MNRAS. A high-resolution HST image of SN 2014J in M82 is available upon reques

Measurement of Protein Tyrosine Phosphatase Activity in Single Cells by Capillary Electrophoresis

A fluorescent peptide substrate was used to measure dephosphorylation by protein tyrosine phosphatases (PTP) in cell lysates, and single cells and to investigate the effect of environmental toxins on PTP activity in these systems. Dephosphorylation of the substrate by PTPN1 and PTPN2 obeyed Michaelis-Menten kinetics, with KM values of 770 ± 250 nM and 290 ± 54 nM, respectively. Dose-response curves and IC50 values were determined for the inhibition of these two enzymes by the environmental toxins Zn2+ and 1,2-naphthoquinone, as well as pervanadate. In A431 cell lysates, the reporter was a poor substrate for peptidases (degradation rate of 100 ± 8.2 fmol min−1 mg−1) but an excellent substrate for phosphatases (dephosphorylation rate of 1.4 ± 0.3 nmol min−1 mg−1). Zn2+, 1,2-naphthoquinone and pervanadate inhibited dephosphorylation of the reporter in cell lysates with IC50 values of 470 nM, 35 μM, and 100 nM, respectively. Dephosphorylation of the reporter following loading into living single cells occurred at rates of at least 2 pmol min−1 mg−1. When single cells were exposed to 1,2-naphthoquinone (50 μM), Zn2+ (100 μM), and pervandate (1 mM), dephosphorylation was inhibited with median values and first and third quartile values of 41 (Q1 = 0%, Q3 = 96%), 50 (Q1 = 46%, Q3 = 74%), and 53% (Q1 = 36%, Q3 = 77%), respectively, demonstrating both the impact of these toxic exposures on cell signaling and the heterogeneity of response between cells. This approach will provide a valuable tool for the study of PTP dynamics, particularly in small, heterogeneous populations such as human biopsy specimens